![]() Rico, Andreu Dimitrov, Mauricio R Van Wijngaarden, René P A Satapornvanit, Kriengkrai Smidt, Hauke Van den Brink, Paul J Copyright © 2011 SETAC.Įffects of the antibiotic enrofloxacin on the ecology of tropical eutrophic freshwater microcosms. In contrast, for enrofloxacin no risk was identified at predicted and measured concentrations. For ciprofloxacin the results of the present study indicate a risk even at the predicted environmental concentration. For two of the four species, a risk was identified at ciprofloxacin concentrations found in surface waters, sewage treatment plant influents and effluents, as well as in hospital effluents. These data, as well as effect data from the literature, were compared with predicted and reported environmental concentrations. The enrofloxacin/ciprofloxacin concentration as measured by AUC in DS samples was 137 ± 72% higher than in plasma, but in BAL samples, this value was 535 ± 403% (p 8,042 µg/L for enrofloxacin and ciprofloxacin, respectively. Pharmacokinetic analysis was performed on the concentrations in each fluid, for each drug. PELF, sampled by two different methods-bronchoalveolar lavage (BAL) and direct sampling (DS)-plasma, and ISF were collected from each calf and measured for tilmicosin, enrofloxacin and its metabolite ciprofloxacin by HPLC. Enrofloxacin ( Baytril ® 100) was administered at a dose of 12.5 mg/kg subcutaneously (SC), and tilmicosin (Micotil ® 300) was administered at a dose of 20 mg/kg SC. All rights reserved.Ĭomparison of direct sampling and brochoalveolar lavage for determining active drug concentrations in the pulmonary epithelial lining fluid of calves injected with enrofloxacin or tilmicosin.Īntibiotic distribution to interstitial fluid (ISF) and pulmonary epithelial fluid (PELF) was measured and compared to plasma drug concentrations in eight healthy calves. This article is part of a Special Issue entitled: HUPO 2014. All 2D DIGE and MS data have been deposited into the ProteomeXchange Consortium with identifier PXD002000 and DOI. In parallel, the dramatic decrease of the synthesis of the outer membrane protein W, which represents one of the main gates for enrofloxacin entrance, could explain additional mechanism of E. ![]() Moreover, since enrofloxacin is an inhibitor of DNA gyrase, the overexpression of DNA starvation/stationary phase protection protein (Dsp) could be a central point to discover the mechanism of this clone to counteract the effects of enrofloxacin. Discovered differentially expressed proteins are principally involved in antibiotic resistance and linked to oxidative stress response, to DNA protection and to membrane permeability. coli has been induced with enrofloxacin and studied through 2D DIGE and shotgun MS. coli isolates that represent a good tool to study this pathology. This study has been performed in order to unravel the mechanism of induced enrofloxacin resistance in canine E. coli) urinary tract infections (UTIs) are becoming a serious problem both for pets and humans (zoonosis) due to the close contact and to the increasing resistance to antibiotics. Piras, Cristian Soggiu, Alessio Greco, Viviana Martino, Piera Anna Del Chierico, Federica Putignani, Lorenza Urbani, Andrea Nally, Jarlath E Bonizzi, Luigi Roncada, PaolaĮscherichia coli (E. Mechanisms of antibiotic resistance to enrofloxacin in uropathogenic Escherichia coli in dog.
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